This enzyme catalyses the following chemical reaction, UDP-N-acetylglucosamine---decaprenyl-phosphate N-acetylglucosaminephosphotransferase is an enzyme with systematic name UDP-N-acetyl-alpha-D-glucosamine:trans,octacis-decaprenyl-phosphate N-acetylglucosaminephosphotransferase. We use cookies to help provide and enhance our service and tailor content and ads. Gerald Litwack Ph.D., in Human Biochemistry, 2018. UDP-N-acetylglucosamine—peptide N-acetylglucosaminyltransferase, also known as O-linked β-N-acetylglucosamine transferase and O-GlcNAc transferase, OGT is an enzyme that in humans is encoded by the OGT gene. UDP-N-acetylglucosamine—undecaprenyl-phosphate N-acetylglucosaminephosphotransferase is an enzyme with systematic name UDP-N-acetyl-alpha-D-glucosamine:ditrans,octacis-undecaprenyl phosphate N-acetyl-alpha-D-glucosaminephosphotransferase. It is used by glycosyltransferases to transfer N-acetylglucosamine residues to substrates. More recent studies have applied the pharmacophore analysis to specific UGT isoforms. 2002a) Collier et al. Diagram showing the transporter (antiporter) for UMP and UDP-Gal. Figure 6.33. ; the enzyme is stored in 20 mM K+–HEPES, 1 mM EDTA, 2 mM dithiothreitol (DTT), 15% (v/v) glycerol, pH 7.2.

Semi-empirical charge calculations performed clearly better than fully empirical ones. [4], UDP-GlcNAc is extensively involved in intracellular signaling as a substrate for O-linked N-acetylglucosamine transferases (OGTs) in a wide range of species. Little is known about the UGTs and their function in the human placenta, though enzymatic activity has been measured (Collier et al. The exact concentration was calibrated by HPLC (Varian Microsorb 100-5 C18, 4.6 × 250 mm) by coinjection with a known concentration of UDP. respectively. It has been suggested that microglial phagocytosis contributes to the arrangement of brain architecture in the developing mouse brain by clearing apoptotic neuronal cells (Sierra et al., 2010). Peptidoglycan serves a structural role in the bacterial cell wall, giving structural strength, as well as counteracting the osmotic pressure of the cytoplasm. Catecholamine sulfotransferase (SULT1A3) is also involved in catechol metabolism. It facilitates the metabolism and subsequent excretion of potentially toxic xenobiotics, carcinogens, estrogens, bilirubin and therapeutic drugs.

Statistically significant differences in the frequencies of this variant were observed in SI (39.7%), Africans (55.3%), Asians (13.1%), and Caucasians (29.6%). G. Vistoli, A. Pedretti, in Comprehensive Medicinal Chemistry II, 2007, UDP-glucoronosyltransferases (UGTs) are among the most important conjugating enzymes involved in drug-metabolism.

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An improperly folded protein would be degraded by the cytoplasmic proteasome. UDP-Gal and UDP-GalNAc (UDP-N-acetylgalactosamine) are transferred to the Golgi apparatus (Fig. GalNAc is, however, found in the cell surface structure of a few pathogenic bacteria and genes encoding UDP-GlcNAc epimerase have been identified in Pseudomonas aeruginosa,77 E. coli O55:H7,78 and C. jejuni.79 The WbpP enzyme from P. aeruginosa has been shown to be a genuine UDP-GlcNAc epimerase with extremely low UDP-Glc epimerase activity.

In mammals, there is enrichment of OGT transcripts in the pancreas beta-cells, and UDP-GlcNAc is thought to be part of the glucose sensing mechanism.

The 86 kDa protein bound 0.5 mol of UDP-GlcA per mol of protein and, in the presence of UDP-GlcA, bound 0.5 mol NAD+ in a ratio of 0.5 mol mol−1 of catalytic protein.172 The 65 kDa protein was enzymatically inactive, although it was required for stability of the 86 kDa protein.172, Ivar von Kügelgen, T. Kendall Harden, in Advances in Pharmacology, 2011. N-Acetylmannosamine is also known as N-Acetyl-D-mannosamine monohydrate,, N-Acetyl-D-mannosamine which can be abbreviated to ManNAc or, less commonly, NAM). 2002b), though the specific UGT isoform was not identified. The sugar component consists of alternating residues of β-(1,4) linked N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM). [3] The end-product of this pathway is UDP-GlcNAc, which is then used for making glycosaminoglycans, proteoglycans, and glycolipids.

Like CYPs, UGTs exist in multiple enzyme isoforms, differing in substrate specificity.128. N-Acetylmannosamine is a hexosamine monosaccharide. Umamaheswaran Gurusamy, Deepak Gopal Shewade, in Handbook of Pharmacogenomics and Stratified Medicine, 2014. The highest prevalence of (TA) repeats was found in Keralites and Tamilians, which suggests that these two populations are more predisposed to development of cancer, Gilbert syndrome, Crigler-Najjar syndrome and severe toxicity when treated with irinotecan.